Contact an Account ManagerSeparation of natural porphyrins
Summary
Type: HPLC
Application #: 108900
Detection: fluorescence detection, excitation: 400 nm, emission: 600 nm
Conditions: Eluent A: MeOH, 12.5 mM tetrabutylammonium dihydrogen phosphate, pH 6.6
Eluent B: 40 mM NaH2PO4, pH 5.6 in water
Gradient: 10 min 45% A, in 5 min to 75% A, in 5 min to 80% A, in 5 min to 98% A, 15 min 98% A
Flow rate 0.7 ml/min
Authors: Strauss W
Source: Institute for Laser Technology University of Ulm G
Details
Legend: porphyrin standard:
1.) uroporphyrin
2.) heptacarboxyporphyrin
3.) hexacarboxyporphyrin
4.) pentacarboxyporphyrin
5.) coproporphyrin
6. ) mesoporphyrin IX
7.) protoporphyrin.